Composite

Part:BBa_K3077000:Design

Designed by: Wong Annabelle Tin Lam   Group: iGEM19_Hong_Kong_UCCKE   (2019-10-14)


Expression of malS under lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 562
    Illegal AgeI site found at 1727
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

BBa_K1418020 is from E. coli

References

[1] Freundlieb S, Boos W.(1986) a-Amylase of Escherichia coli, Mapping and Cloning of the Structural Gene, mal& and Identification of Its Product as a Periplasmic Protein*. J Biol Chem.261(6):2946-53. [2]